Apoptosis Database

Description [+]

Synonyms: FASLG, FAS LIGAND (TNF SUPERFAMILY, MEMBER 6), FASL, ZGC:162027
Species: Metazoa;Bilateria;Deuterostoma;Chordata;Vertebrata;Pisces; Danio rerio
Short gene description: Fas ligand (TNF superfamily, member 6) [Source:RefSeq_peptide;Acc:NP_001036166]
Family: Death ligand
Process: undefined,
Pathways: extrinsic pathway,
Criteria: manually curated
Curator comment:
Human ortholog(s): FASL
WIKI: FASLG-D_rerio

References [+]

Delineation of the cell-extrinsic apoptosis pathway in the zebrafish.
Eimon PM, Kratz E, Varfolomeev E, Hymowitz SG, Stern H, Zha J, Ashkenazi A
The mammalian extrinsic apoptosis pathway is triggered by Fas ligand (FasL) and Apo2 ligand/tumor necrosis factor (TNF)-related apoptosis-inducing ligand (Apo2L/TRAIL). Ligand binding to cognate receptors activates initiator caspases directly in a death-inducing signaling complex. In Drosophila, TNF ligand binding activates initiator caspases indirectly, through JNK. We characterized the extrinsic pathway in zebrafish to determine how it operates in a nonmammalian vertebrate. We identified homologs of FasL and Apo2L/TRAIL, their receptors, and other components of the cell death machinery. Studies with three Apo2L/TRAIL homologs demonstrated that they bind the receptors zHDR (previously linked to hematopoiesis) and ovarian TNFR (zOTR). Ectopic expression of these ligands during embryogenesis induced apoptosis in erythroblasts and notochord cells. Inhibition of zHDR, zOTR, the adaptor zFADD, or caspase-8-like proteases blocked ligand-induced apoptosis, as did antiapoptotic Bcl-2 family members. Thus, the extrinsic apoptosis pathway in zebrafish closely resembles its mammalian counterpart and cooperates with the intrinsic pathway to trigger tissue-specific apoptosis during embryogenesis in response to ectopic Apo2L/TRAIL expression. Cell Death Differ. 2006 Oct;13(10):1619-30. Epub 2006 Aug 4.

References from Human ortholog(s):

Molecular cloning and expression of the Fas ligand, a novel member of the tumor necrosis factor family.
Suda T, Takahashi T, Golstein P, Nagata S
The Fas antigen (Fas) belongs to the tumor necrosis factor (TNF)/nerve growth factor receptor family, and it mediates apoptosis. Using a soluble form of mouse Fas, prepared by fusion with human immunoglobulin Fc, Fas ligand was detected on the cell surface of a cytotoxic T cell hybridoma, PC60-d10S. A cell population that highly expresses Fas ligand was sorted using a fluorescence-activated cell sorter, and its cDNA was isolated from the sorted cells by expression cloning. The amino acid sequence indicated that Fas ligand is a type II transmembrane protein that belongs to the TNF family. The recombinant Fas ligand expressed in COS cells induced apoptosis in Fas-expressing target cells. Northern hybridization revealed that Fas ligand is expressed in activated splenocytes and thymocytes, consistent with its involvement in T cell-mediated cytotoxicity and in several nonlymphoid tissues, such as testis. Cell. 1993 Dec 17;75(6):1169-78.

Structure & Sequence [+]

Source	Domain Name	Start	End
PFAM A	TNF	145	267

Evolution [+]

View protein alignment and tree with Jalview:

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Homologs list [+]

Name	Relationship	Species
NP_001026730.1	orthology	Chicken
G_gallus_ENSGALP00000034816	orthology	Chicken
FASLG	orthology	Chimpanzee
NP_001092329.1	orthology	Cow
Q9TTJ2_BOVIN	orthology	Cow
NP_001092329.1	orthology	Cow
FASLG	orthology	Dog
FASLG	orthology	Fugu
FASLG	orthology	Horse
FASL	orthology	Human
TNFL6_MACMU	orthology	Macaca
Fasl	orthology	Mouse
FASLG	orthology	Orangutan
FASLG	orthology	Ornithorhynchus
Faslg	orthology	Rat
FASLG	orthology	Tetraodon
FASLG	orthology	Xenopus
FASLG	orthology	Zebra finch
A3RF19_CHICK	paralogy	Chicken
TNFA_PANTR	paralogy	Chimpanzee
TNFSF10	paralogy	Chimpanzee
TNFA_CANFA	paralogy	Dog
TNFSF14	paralogy	Gorilla
TNFA_HORSE	paralogy	Horse
TNF	paralogy	Human
TNFSF10	paralogy	Human
H_sapiens_ENSP00000372988	paralogy	Human
H_sapiens_ENSP00000372790	paralogy	Human
TNFSF14	paralogy	Lyzard
O_latipes_ENSORLP00000020071	paralogy	Medaka
CD40LG	paralogy	Monodelphis
TNF	paralogy	Monodelphis
TRAIL	paralogy	Mouse
TNFSF10	paralogy	Orangutan
TNFSF14	paralogy	Ornithorhynchus
TNFSF15	paralogy	Rabbit
Tnfsf10	paralogy	Rat
CD40LG	paralogy	Xenopus

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Gene Ontology [+]

GO id	Name	Ontology type	Evidence
GO:0006955	immune response	biological_proccess	IEA
GO:0006915	apoptosis	biological_proccess	IEA
GO:0007165	signal transduction	biological_proccess	IEA
GO:0005164	tumor necrosis factor receptor binding	mollecular_function	IEA
GO:0005576	extracellular region	cell_component	IEA
GO:0016020	membrane	cell_component	IEA

KEGG Pathways [+]

Information from other databases [+]

FASLG (Danio rerio)