CASP8 (Mus musculus)
Description [+]
- Synonyms: CASP8, CASPASE 8, CASPASE-8, FLICE, MACH, MCH5
- Species: Metazoa;Bilateria;Deuterostoma;Chordata;Vertebrata;Mammalia;Rodentia; Mus musculus
- Short gene description: caspase 8 Gene [Source:MGI Symbol;Acc:MGI:1261423]
- Family: CASPASE
- Process: apoptosis,
- Pathways: extrinsic pathway,
- Criteria: manually curated
- Curator comment:
- Human ortholog(s): CASP8
- WIKI: CASP8-M_musculus
References [+]
- Targeted disruption of the mouse Caspase 8 gene ablates cell death induction by the TNF receptors, Fas/Apo1, and DR3 and is lethal prenatally.
- Varfolomeev EE, Schuchmann M, Luria V, Chiannilkulchai N, Beckmann JS, Mett IL, Rebrikov D, Brodianski VM, Kemper OC, Kollet O, Lapidot T, Soffer D, Sobe T, Avraham KB, Goncharov T, Holtmann H, Lonai P, Wallach D
- Homozygous targeted disruption of the mouse Caspase 8 (Casp8) gene was found to be lethal in utero. The Caspase 8 null embryos exhibited impaired heart muscle development and congested accumulation of erythrocytes. Recovery of hematopoietic colony-forming cells from the embryos was very low. In fibroblast strains derived from these embryos, the TNF receptors, Fas/Apo1, and DR3 were able to activate the Jun N-terminal kinase and to trigger IkappaB alpha phosphorylation and degradation. They failed, however, to induce cell death, while doing so effectively in wild-type fibroblasts. These findings indicate that Caspase 8 plays a necessary and nonredundant role in death induction by several receptors of the TNF/NGF family and serves a vital role in embryonal development. Immunity. 1998 Aug;9(2):267-76.
- References from Human ortholog(s):
- Involvement of MACH, a novel MORT1/FADD-interacting protease, in Fas/APO-1- and TNF receptor-induced cell death.
- Boldin MP, Goncharov TM, Goltsev YV, Wallach D
- Fas/APO-1 and p55 tumor necrosis factor (TNF) receptor (p55-R) activate cellular mechanisms that result in cell death. Upon activation of these receptors, Fas/APO-1 binds a protein called MORT1 (or FADD) and p55-R binds a protein called TRADD. MORT1 and TRADD can also bind to each other. We have cloned a novel protein, MACH, that binds to MORT1. This protein exists in multiple isoforms, some of which contain a region that has proteolytic activity and shows marked sequence homology to proteases of the ICE/CED-3 family. Cellular expression of the proteolytic MACH isoforms results in cell death. Expression of MACH isoforms that contain an incomplete ICE/CED-3 region provides effective protection against the cytotoxicity induced by Fas/APO-1 or p55-R triggering. These findings suggest that MACH is the most upstream enzymatic component in the Fas/APO-1- and p55-R-induced cell death signaling cascades. Cell. 1996 Jun 14;85(6):803-15.
Structure & Sequence [+]
Pfam domains:
(Pfam is a large collection of protein families.)
| Source | Domain Name | Start | End |
|---|---|---|---|
| PFAM A | DED | 3 | 86 |
| PFAM A | DED | 101 | 183 |
| PFAM A | Peptidase_C14 | 236 | 478 |
Protein sequence [+]
Casp8 | Mus musculus | 10090 | length:480
MDFQSCLYAIAEELGSEDLAALKFLCLDYIPHKKQETIEDAQKLFLRLREKGMLEEGNLS
FLKELLFHISRWDLLVNFLDCNREEMVRELRDPDNAQISPYRVMLFKLSEEVSELELRSF
KFLLNNEIPKCKLEDDLSLLEIFVEMEKRTMLAENNLETLKSICDQVNKSLLGKIEDYER
SSTERRMSLEGREELPPSVLDEMSLKMAELCDSPREQDSESRTSDKVYQMKNKPRGYCLI
INNHDFSKAREDITQLRKMKDRKGTDCDKEALSKTFKELHFEIVSYDDCTANEIHEILEG
YQSADHKNKDCFICCILSHGDKGVVYGTDGKEASIYDLTSYFTGSKCPSLSGKPKIFFIQ
ACQGSNFQKGVPDEAGFEQQNHTLEVDSSSHKNYIPDEADFLLGMATVKNCVSYRDPVNG
TWYIQSLCQSLRERCPQGDDILSILTGVNYDVSNKDDRRNKGKQMPQPTFTLRKKLFFPP
FLKELLFHISRWDLLVNFLDCNREEMVRELRDPDNAQISPYRVMLFKLSEEVSELELRSF
KFLLNNEIPKCKLEDDLSLLEIFVEMEKRTMLAENNLETLKSICDQVNKSLLGKIEDYER
SSTERRMSLEGREELPPSVLDEMSLKMAELCDSPREQDSESRTSDKVYQMKNKPRGYCLI
INNHDFSKAREDITQLRKMKDRKGTDCDKEALSKTFKELHFEIVSYDDCTANEIHEILEG
YQSADHKNKDCFICCILSHGDKGVVYGTDGKEASIYDLTSYFTGSKCPSLSGKPKIFFIQ
ACQGSNFQKGVPDEAGFEQQNHTLEVDSSSHKNYIPDEADFLLGMATVKNCVSYRDPVNG
TWYIQSLCQSLRERCPQGDDILSILTGVNYDVSNKDDRRNKGKQMPQPTFTLRKKLFFPP
Structure links:
Evolution [+]
View protein alignment and tree with Jalview:
Explore tree at phylomeDB: Click here.
Homologs list [+]
| Name | Relationship | Species |
|---|---|---|
| NP_001038154.1 | orthology | Chicken |
| NP_989923.1 | orthology | Chicken |
| CASP8 | orthology | Chimpanzee |
| C_intestinalis_ENSCINP00000024485 | orthology | Ciona |
| NP_001039435.1 | orthology | Cow |
| Q38JA9_CANFA | orthology | Dog |
| T_rubripes_ENSTRUP00000024467 | orthology | Fugu |
| G_aculeatus_ENSGACP00000016969 | orthology | Gasterosteus |
| CASP8 | orthology | Gorilla |
| CASP8 | orthology | Horse |
| CASP8 | orthology | Human |
| CASP8 | orthology | Macaca |
| Q56VC9_ORYLA | orthology | Medaka |
| CASP8 | orthology | Monodelphis |
| Q5RCR7_PONPY | orthology | Orangutan |
| CASP8 | orthology | Ornithorhynchus |
| CASP8 | orthology | Rabbit |
| Casp8 | orthology | Rat |
| T_nigroviridis_ENSTNIP00000010722 | orthology | Tetraodon |
| CASP8 | orthology | Zebra finch |
| casp8 | orthology | Zebrafish |
| A_aegypti_AAEL003444-PA | paralogy | Aedes |
| A_aegypti_AAEL014348-PA | paralogy | Aedes |
| CASPS7 | paralogy | Anopheles |
| CASPS6 | paralogy | Anopheles |
| CASPS5 | paralogy | Anopheles |
| Q90WU0_CHICK | paralogy | Chicken |
| CASP10 | paralogy | Chicken |
| CASP7 | paralogy | Chicken |
| NP_990056.1 | paralogy | Chicken |
| NP_990057.1 | paralogy | Chicken |
| CASP7 | paralogy | Chimpanzee |
| CASP6 | paralogy | Chimpanzee |
| XR_025516.1 | paralogy | Chimpanzee |
| CFLAR | paralogy | Chimpanzee |
| CASP3_PANTR | paralogy | Chimpanzee |
| CASP9 | paralogy | Chimpanzee |
| C_intestinalis_ENSCINP00000003204 | paralogy | Ciona |
| C_intestinalis_ENSCINP00000007986 | paralogy | Ciona |
| C_intestinalis_ENSCINP00000011493 | paralogy | Ciona |
| C_intestinalis_ENSCINP00000011503 | paralogy | Ciona |
| C_intestinalis_ENSCINP00000025743 | paralogy | Ciona |
| IPI00704513.4 | paralogy | Cow |
| IPI00689801.3 | paralogy | Cow |
| CASP6_BOVIN | paralogy | Cow |
| CASP3_BOVIN | paralogy | Cow |
| CASP10 | paralogy | Dog |
| CASP3_CANFA | paralogy | Dog |
| CASP7 | paralogy | Dog |
| CASP6 | paralogy | Dog |
| Dcp-1 | paralogy | Fly |
| decay | paralogy | Fly |
| Ice | paralogy | Fly |
| CASP9 | paralogy | Fugu |
| CASP6 | paralogy | Fugu |
| T_rubripes_ENSTRUP00000024164 | paralogy | Fugu |
| CASP7 | paralogy | Fugu |
| NP_001027871.1 | paralogy | Fugu |
| T_rubripes_ENSTRUP00000044662 | paralogy | Fugu |
| G_aculeatus_ENSGACP00000005791 | paralogy | Gasterosteus |
| CASP9 | paralogy | Gasterosteus |
| CASP7 | paralogy | Gasterosteus |
| G_aculeatus_ENSGACP00000016983 | paralogy | Gasterosteus |
| CASP3 (4 of 4) | paralogy | Gasterosteus |
| CASP6 | paralogy | Gasterosteus |
| CASP3 (3 of 4) | paralogy | Gasterosteus |
| CASP3 (2 of 4) | paralogy | Gasterosteus |
| CASP3 (1 of 4) | paralogy | Gasterosteus |
| CASP7 | paralogy | Gorilla |
| CASP6 | paralogy | Horse |
| CASP7 | paralogy | Horse |
| CASP9 | paralogy | Horse |
| Q3S2Z5_HORSE | paralogy | Horse |
| CASP10 | paralogy | Horse |
| CASP6 | paralogy | Human |
| CASP10 | paralogy | Human |
| CASP3 | paralogy | Human |
| CFLAR | paralogy | Human |
| CASP7 | paralogy | Human |
| CASP9 | paralogy | Human |
| CASP10 | paralogy | Lyzard |
| CASP3 | paralogy | Lyzard |
| CASP7 | paralogy | Lyzard |
| CASP6 | paralogy | Lyzard |
| CASP7 | paralogy | Macaca |
| Q8SPU2_MACMU | paralogy | Macaca |
| CASP10 | paralogy | Macaca |
| Q8MJ18_MACMU | paralogy | Macaca |
| CASP9 | paralogy | Macaca |
| CASP7 | paralogy | Medaka |
| Q8JIS9_ORYLA | paralogy | Medaka |
| Q8JIS8_ORYLA | paralogy | Medaka |
| O_latipes_ENSORLP00000013993 | paralogy | Medaka |
| CFLAR | paralogy | Monodelphis |
| CASP9 | paralogy | Monodelphis |
| NP_001033059.1 | paralogy | Monodelphis |
| NP_001033061.1 | paralogy | Monodelphis |
| CASP6 | paralogy | Monodelphis |
| CASP10 | paralogy | Monodelphis |
| Casp7 | paralogy | Mouse |
| Casp6 | paralogy | Mouse |
| Casp9 | paralogy | Mouse |
| Casp3 | paralogy | Mouse |
| CASP3 | paralogy | Orangutan |
| CASP9 | paralogy | Orangutan |
| CASP7 | paralogy | Orangutan |
| CFLAR_PONPY | paralogy | Orangutan |
| Q5RB11_PONPY | paralogy | Orangutan |
| CASP6 | paralogy | Orangutan |
| CASP3 | paralogy | Ornithorhynchus |
| O_anatinus_ENSOANP00000001054 | paralogy | Ornithorhynchus |
| CASP7 | paralogy | Ornithorhynchus |
| CASP10 | paralogy | Ornithorhynchus |
| CASP6 | paralogy | Ornithorhynchus |
| CASP9 | paralogy | Ornithorhynchus |
| CASP10 | paralogy | Rabbit |
| CASP7 | paralogy | Rabbit |
| Casp6 | paralogy | Rat |
| Casp3 | paralogy | Rat |
| Casp9 | paralogy | Rat |
| Casp7 | paralogy | Rat |
| T_nigroviridis_ENSTNIP00000001216 | paralogy | Tetraodon |
| CASP3 | paralogy | Tetraodon |
| CASP9 | paralogy | Tetraodon |
| CASP6 | paralogy | Tetraodon |
| CASP7 | paralogy | Tetraodon |
| CASP3 | paralogy | Xenopus |
| casp10 | paralogy | Xenopus |
| casp7 | paralogy | Xenopus |
| casp6 | paralogy | Xenopus |
| T_guttata_ENSTGUP00000004278 | paralogy | Zebra finch |
| CASP3 | paralogy | Zebra finch |
| CASP10 | paralogy | Zebra finch |
| T_guttata_ENSTGUP00000011206 | paralogy | Zebra finch |
| T_guttata_ENSTGUP00000014729 | paralogy | Zebra finch |
| CASP9 | paralogy | Zebra finch |
| casp3a | paralogy | Zebrafish |
| casp7 | paralogy | Zebrafish |
| casp8l2 | paralogy | Zebrafish |
| casp6l1 | paralogy | Zebrafish |
| D_rerio_ENSDARP00000047019 | paralogy | Zebrafish |
| casp6 | paralogy | Zebrafish |
| NP_001077331.1 | paralogy | Zebrafish |
| casp3b | paralogy | Zebrafish |
| A2BGE2_DANRE | paralogy | Zebrafish |
| casp8l1 | paralogy | Zebrafish |
| LOC795066 | paralogy | Zebrafish |
| casp2 | paralogy | Zebrafish |
| casp6l2 | paralogy | Zebrafish |
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Gene Ontology [+]
| GO id | Name | Ontology type | Evidence |
|---|---|---|---|
| GO:0001525 | angiogenesis | biological_proccess | IMP |
| GO:0001841 | neural tube formation | biological_proccess | IMP |
| GO:0006508 | proteolysis | biological_proccess | IEA |
| GO:0007507 | heart development | biological_proccess | IMP |
| GO:0030225 | macrophage differentiation | biological_proccess | IMP |
| GO:0042981 | regulation of apoptosis | biological_proccess | IEA |
| GO:0006915 | apoptosis | biological_proccess | IDA |
| GO:0051291 | protein heterooligomerization | biological_proccess | IEA |
| GO:0043123 | positive regulation of I-kappaB kinase/NF-kappaB cascade | biological_proccess | IEA |
| GO:0034612 | response to tumor necrosis factor | biological_proccess | IEA |
| GO:0051603 | proteolysis involved in cellular protein catabolic process | biological_proccess | IEA |
| GO:0008624 | induction of apoptosis by extracellular signals | biological_proccess | IEA |
| GO:0004197 | cysteine-type endopeptidase activity | mollecular_function | IEA |
| GO:0005515 | protein binding | mollecular_function | IPI |
| GO:0008233 | peptidase activity | mollecular_function | IMP |
| GO:0016787 | hydrolase activity | mollecular_function | IEA |
| GO:0008234 | cysteine-type peptidase activity | mollecular_function | IEA |
| GO:0042802 | identical protein binding | mollecular_function | IEA |
| GO:0005634 | nucleus | cell_component | IDA |
| GO:0005737 | cytoplasm | cell_component | IDA |
| GO:0030690 | Noc1p-Noc2p complex | cell_component | IMP |
| GO:0043234 | protein complex | cell_component | IEA |
| GO:0045121 | membrane raft | cell_component | IEA |
| GO:0005634 | nucleus | cell_component | IEA |
| GO:0031264 | death-inducing signaling complex | cell_component | IEA |
Check GO Evidence Codes here
Information from other databases [+]
- Gene info from MGI [?] MGI:1261423
- Ensembl genome browser [?] : ENSMUSG00000026029
- Expression info from Arrayexpress [?] : ENSMUSG00000026029
- Protein expression from Protein Atlas: [?] ENSMUSG00000026029
- Community gene edition from Wikigenes: [?] 12370
- entrezgene: 12370
- refseq_dna: NM_001080126
- refseq_dna: NM_009812
- refseq_peptide: NP_001073595
- refseq_peptide: NP_033942
Click on [?] for more information.
