BCL2 (Mus musculus)

Description [+]

Synonyms: BCL2, BCL2, B-CELL LEUKEMIA/LYMPHOMA 2
Species: Metazoa;Bilateria;Deuterostoma;Chordata;Vertebrata;Mammalia;Rodentia; Mus musculus
Short gene description: B-cell leukemia/lymphoma 2 Gene [Source:MGI (curated);Acc:Bcl2-001]
Family: Bcl-2 family : multidomain Bcl-2
Process: apoptosis,
Pathways: intrinsic pathway, pre-mitochondrial signaling events,
Criteria: manually curated
Curator comment:
Human ortholog(s): BCL2
WIKI: BCL2-M_musculus

References [+]

Molecular analysis of mbcl-2: structure and expression of the murine gene homologous to the human gene involved in follicular lymphoma.
Negrini M, Silini E, Kozak C, Tsujimoto Y, Croce CM
We have cloned the mouse bcl-2 (mbcl-2) genomic locus and analyzed it in detail. The gene is comprised of two exons separated by more than 15 kb. Two species of mRNAs are produced, and DNA sequencing analysis shows that they code for two proteins differing at their C terminus: a 7.5 kb transcript codes for a polypeptide of 236 amino acids, mbcl-2 alpha, and a 2.4 kb transcript, which derives from the 5' exon only, codes for a protein of 199 amino acids, mbcl-2 beta. The gene is characterized by very long (5' about 1.4 kb, and 3' about 5.1 kb) untranslated regions surrounding the relatively short coding region. We have mapped the 5' end of the mbcl-2 mRNAs by S1 protection analysis, and we have analyzed the promoter region. The expression of the mbcl-2 gene was analyzed in different cell lines and in mouse tissues. Expression is tissue-specific in adult tissues: spleen and thymus express the highest level of mbcl-2 transcripts. The mbcl-2 gene maps to mouse chromosome 1. Cell. 1987 May 22;49(4):455-63.

References from Human ortholog(s):

Oncogenic potential of bcl-2 demonstrated by gene transfer.
Reed JC, Cuddy M, Slabiak T, Croce CM, Nowell PC
Follicular lymphoma is the most common human B-cell malignancy in the United States and Western Europe. Most of the tumours contain t(14;18) chromosome translocations involving the human bcl-2 gene. Translocation of bcl-2 sequences from chromosome 18 into the transcriptionally active immunoglobulin locus at chromosome band 14q32 in B cells deregulates bcl-2 gene expression, resulting in the accumulation of high levels of bcl-2 messenger. Human bcl-2 transcripts generate two proteins, p26 bcl-2-alpha and p22 bcl-2-beta, by virtue of alternative splice-site selection. Both proteins have in common their first 196 NH2-terminal amino acids but share little similarity with other sequences in a data bank. Although the biological and biochemical functions of bcl-2 are unknown, recent subcellular localization studies indicate that p26 bcl-2-alpha associates with cellular membranes, consistent with a stretch of hydrophobic amino acids in its carboxy terminus. The bcl-2 gene may represent a novel oncogene having no known retroviral counterpart. Here we demonstrate the oncogenic potential of bcl-2 through a gene transfer approach. Nature. 1988 Nov 17;336(6196):259-61.

Structure & Sequence [+]

Pfam domains: (Pfam is a large collection of protein families.)

Source	Domain Name	Start	End
PFAM A	BH4	7	33
PFAM A	Bcl-2	94	192

Protein sequence [+]

Structure links:

Smartdomain prediction information: SM00265
Smartdomain prediction information: SM00337
Prosite motif and domain information: PS01080
Prosite motif and domain information: PS01258
Prosite motif and domain information: PS01259
Prosite motif and domain information: PS01260
Interpro domain information: P10417
PFAM domain and domain family information: P10417

Evolution [+]

Name	Relationship	Species
BCL2_CHICK	orthology	Chicken
BCL2	orthology	Chimpanzee
Q462R3_BOVIN	orthology	Cow
BCL2	orthology	Dog
BCL2	orthology	Fugu
BCL2	orthology	Gasterosteus
BCL2	orthology	Gorilla
BCL2	orthology	Horse
BCL2	orthology	Human
BCL2	orthology	Lyzard
BCL2	orthology	Macaca
BCL2	orthology	Medaka
BCL2	orthology	Monodelphis
BCL2	orthology	Orangutan
Bcl2	orthology	Rat
BCL2	orthology	Tetraodon
BCL2	orthology	Xenopus
BCL2	orthology	Zebra finch
bcl2	orthology	Zebrafish
A_aegypti_AAEL001521-PA	paralogy	Aedes
A_aegypti_AAEL001515-PA	paralogy	Aedes
Q2PHG8_ANOGA	paralogy	Anopheles
NP_001026091.1	paralogy	Chicken
BCLX_CHICK	paralogy	Chicken
BCL2L1	paralogy	Chimpanzee
XR_024448.1	paralogy	Chimpanzee
BAX	paralogy	Chimpanzee
BCL2A1	paralogy	Chimpanzee
MCL1	paralogy	Chimpanzee
BCL2L2	paralogy	Chimpanzee
C_intestinalis_ENSCINP00000000654	paralogy	Ciona
C_intestinalis_ENSCINP00000024813	paralogy	Ciona
C_intestinalis_ENSCINP00000019812	paralogy	Ciona
NP_001092676.1	paralogy	Cow
BAX_BOVIN	paralogy	Cow
BCLW_BOVIN	paralogy	Cow
B2LA1_BOVIN	paralogy	Cow
IPI00908204.1	paralogy	Cow
NP_001070954.1	paralogy	Cow
NP_001071386.1	paralogy	Cow
NP_001018644.1	paralogy	Dog
NP_001003072.1	paralogy	Dog
MCL1_CANFA	paralogy	Dog
BCL2A1	paralogy	Dog
debcl	paralogy	Fly
Buffy	paralogy	Fly
BCL2L1 (5 of 6)	paralogy	Fugu
BCL2L1 (4 of 6)	paralogy	Fugu
MCL1	paralogy	Fugu
BCL2L1 (1 of 6)	paralogy	Fugu
BCL2L1 (6 of 6)	paralogy	Fugu
BAX	paralogy	Fugu
BCL2L1 (3 of 6)	paralogy	Fugu
BCL2L1 (2 of 6)	paralogy	Fugu
T_rubripes_ENSTRUP00000006891	paralogy	Fugu
BAX	paralogy	Gasterosteus
MCL1	paralogy	Gasterosteus
BCL2L1 (2 of 2)	paralogy	Gasterosteus
BCL2L1 (1 of 2)	paralogy	Gasterosteus
G_aculeatus_ENSGACP00000018978	paralogy	Gasterosteus
BCL2L2	paralogy	Gorilla
BAK1	paralogy	Gorilla
BAX	paralogy	Gorilla
MCL1	paralogy	Gorilla
BOK	paralogy	Gorilla
BAK1	paralogy	Horse
BCL2L2	paralogy	Horse
BCL2L1	paralogy	Horse
BCL2A1	paralogy	Horse
MCL1	paralogy	Horse
BCL2L1	paralogy	Human
BOK	paralogy	Human
BCL2L2	paralogy	Human
BCL2A1	paralogy	Human
MCL1	paralogy	Human
BAX	paralogy	Human
BAK1	paralogy	Human
BAX	paralogy	Lyzard
BOK	paralogy	Lyzard
BAK1	paralogy	Lyzard
MCL1	paralogy	Lyzard
A_carolinensis_ENSACAP00000004550	paralogy	Lyzard
BCL2L1	paralogy	Lyzard
BCL2L2	paralogy	Macaca
BOK	paralogy	Macaca
BAK1	paralogy	Macaca
BCL2L1	paralogy	Macaca
MCL1	paralogy	Macaca
BAX	paralogy	Macaca
BCL2A1	paralogy	Macaca
MCL1	paralogy	Medaka
BCL2L1	paralogy	Medaka
BAX	paralogy	Monodelphis
BOK	paralogy	Monodelphis
MCL1	paralogy	Monodelphis
BCL2A1	paralogy	Monodelphis
M_domestica_ENSMODP00000037249	paralogy	Monodelphis
M_domestica_ENSMODP00000005598	paralogy	Monodelphis
BAK1	paralogy	Monodelphis
BCL2L1	paralogy	Monodelphis
Bax	paralogy	Mouse
Bcl2a1d	paralogy	Mouse
Bcl2a1a	paralogy	Mouse
Bok	paralogy	Mouse
Bcl2a1a	paralogy	Mouse
Bcl2l2	paralogy	Mouse
Mcl1	paralogy	Mouse
Bcl2l1	paralogy	Mouse
Bak1	paralogy	Mouse
MCL1	paralogy	Orangutan
BCL2A1	paralogy	Orangutan
BOK	paralogy	Orangutan
P_pygmaeus_ENSPPYP00000006150	paralogy	Orangutan
BCL2L1	paralogy	Orangutan
BCL2L2	paralogy	Ornithorhynchus
BOK	paralogy	Ornithorhynchus
Q9MYW4_RABIT	paralogy	Rabbit
MCL1	paralogy	Rabbit
BAK1	paralogy	Rabbit
BAX	paralogy	Rabbit
BCL2A1	paralogy	Rabbit
Bcl2a1	paralogy	Rat
LOC684140	paralogy	Rat
BCLX_RAT	paralogy	Rat
Bok	paralogy	Rat
RGD1565822_predicted	paralogy	Rat
Bax	paralogy	Rat
Bak1	paralogy	Rat
BCL2L1 (3 of 3)	paralogy	Tetraodon
BOK (1 of 2)	paralogy	Tetraodon
BCL2L1 (1 of 3)	paralogy	Tetraodon
T_nigroviridis_ENSTNIP00000017211	paralogy	Tetraodon
BCL2L1 (2 of 3)	paralogy	Tetraodon
bcl2l2	paralogy	Xenopus
BAK1	paralogy	Xenopus
MCL1	paralogy	Xenopus
bax	paralogy	Xenopus
BCL2L1	paralogy	Xenopus
BAX	paralogy	Zebra finch
BCL2A1	paralogy	Zebra finch
BCL2L1	paralogy	Zebra finch
BOK	paralogy	Zebra finch
baxa	paralogy	Zebrafish
mcl1a	paralogy	Zebrafish
mcl1b	paralogy	Zebrafish
bcl2l	paralogy	Zebrafish
D_rerio_ENSDARP00000037180	paralogy	Zebrafish

Gene Ontology [+]

GO id	Name	Ontology type	Evidence
GO:0000082	G1/S transition of mitotic cell cycle	biological_proccess	IDA
GO:0000902	cell morphogenesis	biological_proccess	IMP
GO:0001101	response to acid	biological_proccess	IDA
GO:0001541	ovarian follicle development	biological_proccess	IMP
GO:0001658	ureteric bud branching	biological_proccess	IGI
GO:0001662	behavioral fear response	biological_proccess	IMP
GO:0001666	response to hypoxia	biological_proccess	IEA
GO:0001782	B cell homeostasis	biological_proccess	IMP
GO:0001952	regulation of cell-matrix adhesion	biological_proccess	IMP
GO:0002320	lymphoid progenitor cell differentiation	biological_proccess	IMP
GO:0002326	B cell lineage commitment	biological_proccess	IMP
GO:0002360	T cell lineage commitment	biological_proccess	IMP
GO:0002520	immune system development	biological_proccess	IMP
GO:0003014	renal system process	biological_proccess	IMP
GO:0006470	protein amino acid dephosphorylation	biological_proccess	IDA
GO:0006582	melanin metabolic process	biological_proccess	IMP
GO:0006800	oxygen and reactive oxygen species metabolic process	biological_proccess	IMP
GO:0006808	regulation of nitrogen utilization	biological_proccess	IGI
GO:0006916	anti-apoptosis	biological_proccess	IMP
GO:0006979	response to oxidative stress	biological_proccess	IMP
GO:0007015	actin filament organization	biological_proccess	IMP
GO:0007569	cell aging	biological_proccess	IMP
GO:0007584	response to nutrient	biological_proccess	IEA
GO:0008219	cell death	biological_proccess	IMP
GO:0008584	male gonad development	biological_proccess	IGI
GO:0009408	response to heat	biological_proccess	IEA
GO:0009605	response to external stimulus	biological_proccess	IMP
GO:0009636	response to toxin	biological_proccess	IMP
GO:0009791	post-embryonic development	biological_proccess	IMP
GO:0009887	organ morphogenesis	biological_proccess	IMP
GO:0010035	response to inorganic substance	biological_proccess	IEA
GO:0010224	response to UV-B	biological_proccess	IMP
GO:0010332	response to gamma radiation	biological_proccess	IMP
GO:0010468	regulation of gene expression	biological_proccess	IMP
GO:0010523	negative regulation of calcium ion transport into cytosol	biological_proccess	IGI
GO:0010559	regulation of glycoprotein biosynthetic process	biological_proccess	IMP
GO:0014031	mesenchymal cell development	biological_proccess	IMP
GO:0014042	positive regulation of neuron maturation	biological_proccess	IMP
GO:0014911	positive regulation of smooth muscle cell migration	biological_proccess	IMP
GO:0016337	cell-cell adhesion	biological_proccess	IMP
GO:0018107	peptidyl-threonine phosphorylation	biological_proccess	IDA
GO:0021747	cochlear nucleus development	biological_proccess	IMP
GO:0022612	gland morphogenesis	biological_proccess	IMP
GO:0030097	hemopoiesis	biological_proccess	IMP
GO:0030279	negative regulation of ossification	biological_proccess	IMP
GO:0030308	negative regulation of cell growth	biological_proccess	IMP
GO:0030336	negative regulation of cell migration	biological_proccess	IMP
GO:0031000	response to caffeine	biological_proccess	IEA
GO:0031103	axon regeneration	biological_proccess	IDA
GO:0031647	regulation of protein stability	biological_proccess	IMP
GO:0032469	endoplasmic reticulum calcium ion homeostasis	biological_proccess	IGI
GO:0032835	glomerulus development	biological_proccess	IMP
GO:0032880	regulation of protein localization	biological_proccess	IMP
GO:0033033	negative regulation of myeloid cell apoptosis	biological_proccess	IDA
GO:0033077	T cell differentiation in the thymus	biological_proccess	IMP
GO:0033138	positive regulation of peptidyl-serine phosphorylation	biological_proccess	IGI
GO:0033689	negative regulation of osteoblast proliferation	biological_proccess	IMP
GO:0034097	response to cytokine stimulus	biological_proccess	IMP
GO:0035265	organ growth	biological_proccess	IMP
GO:0040018	positive regulation of multicellular organism growth	biological_proccess	IMP
GO:0042493	response to drug	biological_proccess	IDA
GO:0042542	response to hydrogen peroxide	biological_proccess	IMP
GO:0043029	T cell homeostasis	biological_proccess	IGI
GO:0043085	positive regulation of catalytic activity	biological_proccess	IMP
GO:0043375	CD8-positive, alpha-beta T cell lineage commitment	biological_proccess	IMP
GO:0043434	response to peptide hormone stimulus	biological_proccess	IEA
GO:0043524	negative regulation of neuron apoptosis	biological_proccess	IDA
GO:0043583	ear development	biological_proccess	IMP
GO:0043627	response to estrogen stimulus	biological_proccess	IEA
GO:0045471	response to ethanol	biological_proccess	IEA
GO:0045636	positive regulation of melanocyte differentiation	biological_proccess	IMP
GO:0045930	negative regulation of mitotic cell cycle	biological_proccess	IDA
GO:0046671	negative regulation of retinal cell programmed cell death	biological_proccess	IMP
GO:0048041	focal adhesion formation	biological_proccess	IMP
GO:0048087	positive regulation of pigmentation during development	biological_proccess	IMP
GO:0048536	spleen development	biological_proccess	IGI
GO:0048538	thymus development	biological_proccess	IMP
GO:0048547	gut morphogenesis	biological_proccess	IMP
GO:0048589	developmental growth	biological_proccess	IMP
GO:0048599	oocyte development	biological_proccess	IMP
GO:0048743	positive regulation of skeletal muscle fiber development	biological_proccess	IMP
GO:0048753	pigment granule organization	biological_proccess	IMP
GO:0048873	homeostasis of number of cells within a tissue	biological_proccess	IMP
GO:0051384	response to glucocorticoid stimulus	biological_proccess	IMP
GO:0051726	regulation of cell cycle	biological_proccess	IMP
GO:0051789	response to protein stimulus	biological_proccess	IMP
GO:0001836	release of cytochrome c from mitochondria	biological_proccess	IDA
GO:0008284	positive regulation of cell proliferation	biological_proccess	IDA
GO:0031069	hair follicle morphogenesis	biological_proccess	IMP
GO:0045069	regulation of viral genome replication	biological_proccess	IDA
GO:0046902	regulation of mitochondrial membrane permeability	biological_proccess	IDA
GO:0051881	regulation of mitochondrial membrane potential	biological_proccess	IDA
GO:0007409	axonogenesis	biological_proccess	IMP
GO:0018105	peptidyl-serine phosphorylation	biological_proccess	IDA
GO:0000209	protein polyubiquitination	biological_proccess	ISO
GO:0006874	cellular calcium ion homeostasis	biological_proccess	IDA
GO:0001503	ossification	biological_proccess	IMP
GO:0001656	metanephros development	biological_proccess	IMP
GO:0042221	response to chemical stimulus	biological_proccess	IDA
GO:0001657	ureteric bud development	biological_proccess	IMP
GO:0030307	positive regulation of cell growth	biological_proccess	ISO
GO:0042100	B cell proliferation	biological_proccess	ISO
GO:0006915	apoptosis	biological_proccess	IDA
GO:0008285	negative regulation of cell proliferation	biological_proccess	IMP
GO:0016049	cell growth	biological_proccess	IMP
GO:0040007	growth	biological_proccess	IMP
GO:0030318	melanocyte differentiation	biological_proccess	IMP
GO:0030183	B cell differentiation	biological_proccess	IMP
GO:0008283	cell proliferation	biological_proccess	IGI
GO:0030217	T cell differentiation	biological_proccess	IMP
GO:0048066	pigmentation during development	biological_proccess	IMP
GO:0048545	response to steroid hormone stimulus	biological_proccess	IMP
GO:0001776	leukocyte homeostasis	biological_proccess	IMP
GO:0043066	negative regulation of apoptosis	biological_proccess	IDA
GO:0050790	regulation of catalytic activity	biological_proccess	IMP
GO:0001822	kidney development	biological_proccess	IGI
GO:0042981	regulation of apoptosis	biological_proccess	IGI
GO:0048070	regulation of pigmentation during development	biological_proccess	IGI
GO:0043067	regulation of programmed cell death	biological_proccess	IGI
GO:0051924	regulation of calcium ion transport	biological_proccess	ISO
GO:0051402	neuron apoptosis	biological_proccess	IMP
GO:0002260	lymphocyte homeostasis	biological_proccess	IGI
GO:0070059	apoptosis in response to endoplasmic reticulum stress	biological_proccess	ISS
GO:0042493	response to drug	biological_proccess	IEA
GO:0006916	anti-apoptosis	biological_proccess	IEA
GO:0043524	negative regulation of neuron apoptosis	biological_proccess	IEA
GO:0010039	response to iron ion	biological_proccess	IEA
GO:0043497	regulation of protein heterodimerization activity	biological_proccess	IEA
GO:0034097	response to cytokine stimulus	biological_proccess	IEA
GO:0008219	cell death	biological_proccess	IEA
GO:0009636	response to toxin	biological_proccess	IEA
GO:0070059	apoptosis in response to endoplasmic reticulum stress	biological_proccess	IEA
GO:0035094	response to nicotine	biological_proccess	IEA
GO:0051607	defense response to virus	biological_proccess	IEA
GO:0043496	regulation of protein homodimerization activity	biological_proccess	IEA
GO:0051924	regulation of calcium ion transport	biological_proccess	IEA
GO:0042100	B cell proliferation	biological_proccess	IEA
GO:0032848	negative regulation of cellular pH reduction	biological_proccess	IEA
GO:0046688	response to copper ion	biological_proccess	IEA
GO:0051412	response to corticosterone stimulus	biological_proccess	IEA
GO:0010044	response to aluminum ion	biological_proccess	IEA
GO:0051593	response to folic acid	biological_proccess	IEA
GO:0008134	transcription factor binding	mollecular_function	IPI
GO:0016563	transcription activator activity	mollecular_function	IDA
GO:0046982	protein heterodimerization activity	mollecular_function	IPI
GO:0005515	protein binding	mollecular_function	IPI
GO:0042803	protein homodimerization activity	mollecular_function	IEA
GO:0046982	protein heterodimerization activity	mollecular_function	IEA
GO:0002020	protease binding	mollecular_function	IEA
GO:0051434	BH3 domain binding	mollecular_function	IEA
GO:0000159	protein phosphatase type 2A complex	cell_component	IDA
GO:0005622	intracellular	cell_component	IDA
GO:0005624	membrane fraction	cell_component	IDA
GO:0005634	nucleus	cell_component	IDA
GO:0005737	cytoplasm	cell_component	IDA
GO:0005739	mitochondrion	cell_component	IDA
GO:0005783	endoplasmic reticulum	cell_component	IDA
GO:0005789	endoplasmic reticulum membrane	cell_component	IDA
GO:0005792	microsome	cell_component	IDA
GO:0005829	cytosol	cell_component	IDA
GO:0005955	calcineurin complex	cell_component	IDA
GO:0016020	membrane	cell_component	IEA
GO:0031965	nuclear membrane	cell_component	IDA
GO:0031966	mitochondrial membrane	cell_component	IDA
GO:0043209	myelin sheath	cell_component	IDA
GO:0005741	mitochondrial outer membrane	cell_component	IEA
GO:0016021	integral to membrane	cell_component	IEA
GO:0005737	cytoplasm	cell_component	IEA
GO:0005634	nucleus	cell_component	IEA
GO:0005739	mitochondrion	cell_component	IEA
GO:0031965	nuclear membrane	cell_component	IEA

Check GO Evidence Codes here

KEGG Pathways [+]

miRNAs [+]

miRNA	Regulation	Description	Pubmed
mmu-miR-181a	siRNA anti-miRNA	The 3_ UTR elements of several predicted targets of miR-181a were fused to a luciferase reporter, and we examined the ability of a synthetic miR-181a small interfering RNA (siRNA) duplex to repress the expression of、 these constructs in HeLa cells. Relative to transfection of a control siRNA, miR-181a significantly down-regulated reporters fused to the 3_ UTRs of the TCR_ chain, CD69, and Bcl2 (Fig. 4).	Ref.

Curated Isoforms [+]

Transcript	Translation
OTTMUST00000052517 *	OTTMUSP00000024915 *

Information from other databases [+]

Gene related links:

Gene info from MGI [?] MGI:88138
Ensembl genome browser [?] : ENSMUSG00000057329
Expression info from Arrayexpress [?] : ENSMUSG00000057329
Protein expression from Protein Atlas: [?] ENSMUSG00000057329
Community gene edition from Wikigenes: [?] 12043

Protein related links:

Uniprot protein info: P10417
PIR protein info: P10417
Protein interactions from IntAct: P10417

Other links

entrezgene: 12043
refseq_dna: NM_009741
refseq_dna: NM_177410
refseq_peptide: NP_803129
refseq_peptide: NP_033871

Click on [?] for more information.